CD11b/c Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CD11b\/Integrin \u03b1M\/Integrin Alpha M Polyclonal Antibody, Unconjugated (bs-1014R) at 1:300, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse brain tissue labeled with Anti-CD11b\/Integrin \u03b1M\/Integrin Alpha M Polyclonal Antibody, Unconjugated (bs-1014R) at 1:300, followed by conjugation to the secondary antibody and DAB staining

$Human U937 cells probed with CD11b\/c Polyclonal Antibody, Unconjugated (bs-1014R ) (0.2ug) for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control unstained cells (blue), secondary only (light blue) and isotype control (orange).$

Human U937 cells probed with CD11b\/c Polyclonal Antibody, Unconjugated (bs-1014R ) (0.2ug) for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control unstained cells (blue), secondary only (light blue) and isotype control (orange).

$This image was kindly submitted by Kandy Velazquez from USC School of Medicine.\r\nMouse colon lysates probed with CD11b\/c Polyclonal Antibody, unconjugated (bs-1014R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at 37\u00b0C.$

This image was kindly submitted by Kandy Velazquez from USC School of Medicine.\r\nMouse colon lysates probed with CD11b\/c Polyclonal Antibody, unconjugated (bs-1014R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:2000 for 60 minutes at 37\u00b0C.

$Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Rat Spleen tissue lysates probed with CD11b Polyclonal Antibody, Unconjugated (bs-1014R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Lymph node tissue lysates; Lane 2: Mouse Spleen tissue lysates; Lane 3: Rat Spleen tissue lysates probed with CD11b Polyclonal Antibody, Unconjugated (bs-1014R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-1014R
Product Name	CD11b/c Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	The immunogen of this product has partial sequence similarity to CD11d and may cross react.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD11b
Immunogen Range	77-180/1152
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3684
Swiss Prot	P11215
Subcellular location	Cell membrane
Synonyms	CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; ITGAM; Integrin alpha-X; ITGAX; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD11c
Background	Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200