PLAG1 Polyclonal Antibody

$Lane 1: mouse lung lysates probed with \\tPLAG1 Polyclonal Antibody, Unconjugated (bs-10092R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.$

Lane 1: mouse lung lysates probed with \\tPLAG1 Polyclonal Antibody, Unconjugated (bs-10092R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-10092R
Product Name	PLAG1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLAG1
Immunogen Range	51-150/500
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5324
Swiss Prot	Q6DJT9
Subcellular location	Nucleus
Synonyms	PSA; SGPA; ZNF912; Zinc finger protein PLAG1; Pleiomorphic adenoma gene 1 protein; PLAG1
Background	Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000