ROR1 Polyclonal Antibody

$Antigen: bs-10067P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-10067P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Lane 1:A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: MDA-MB-231 cell lysates probed with ROR1 Polyclonal Antibody, Unconjugated (bs-10076R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Rat
Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-10076R
Product Name	ROR1 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Predicted Reactivity	Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ROR1
Immunogen Range	301-360/937
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4919
Swiss Prot	Q01973
Subcellular location	Cell membrane
Synonyms	NTRKR1; dJ537F10.1; Tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase, receptor-related 1; ROR1
Background	Tyrosine-protein kinase receptor whose role is not yet clear. ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000