Alpha s2 Casein Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded bovine breast tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-alpha S2 casein Polyclonal Antibody, Unconjugated (bs-10034R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded bovine breast tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-alpha S2 casein Polyclonal Antibody, Unconjugated (bs-10034R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F

Reactivity

Mouse
Bovine

Predicted Reactivity

Cow
Sheep

Overview
Catalog #	bs-10034R
Product Name	Alpha s2 Casein Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F
Reactivity	Mouse, Bovine
Predicted Reactivity	Cow, Sheep
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from cow Alpha s2 Casein
Immunogen Range	165-203/222
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	282209
Swiss Prot	P02663
Subcellular location	Secreted
Synonyms	Alpha-S2-casein; CSN1S2
Background	Important role in the capacity of milk to transport calcium phosphate. Casocidin-I inhibits the growth of E.coli and S.carnosus.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500