Glycophorin A Polyclonal Antibody

$Antigen: bs-10017P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n$

Antigen: bs-10017P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n

$Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates probed with Glycophorin A Polyclonal Antibody, Unconjugated (bs-11261R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HL-60 cell lysates; Lane 2: Human MOLT4 cell lysates probed with Glycophorin A Polyclonal Antibody, Unconjugated (bs-11261R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human

Overview
Catalog #	bs-10017R
Product Name	Glycophorin A Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD235a/Glycophorin A
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1502173
Subcellular location	Extracellular
Synonyms	Glycophorin A; AI853584; CD235a; GPA; Blood group-MN locus; CD235a antigen; Glycophorin A MNS blood group; Glycophorin A includes MN blood group; Glycophorin A precursor; Glycophorin A, included; GlycophorinA; GPA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HgpMiV; HgpMiX; HgpMiXI; HGpStaC; MN; MN sialoglycoprotein; MNS; PAS 2; PAS-2; PAS2;Sialoglycoprotein alpha.
Background	Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors and also binds influenza virus.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200