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Nanog Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Pig
Overview
Catalog # bs-0829R-PE
Product Name Nanog Polyclonal Antibody, PE Conjugated
Applications WB, FCM
Reactivity Human, Mouse
Predicted Reactivity Rat, Pig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Nanog
Immunogen Range 71-170/305
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 71950
Swiss Prot Q80Z64
Subcellular location Nucleus
Synonyms ENK; ecat4; 2412E2Rik; Homeobox protein NANOG; ES cell-associated protein 4; Early embryo specific expression NK-type homeobox protein; Homeobox transcription factor Nanog; Nanog
Background Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Application Dilution
WB 1:300-5000
FCM 1:20-100