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Collagen 4 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Rabbit

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-0806R-Cy7
Product Name Collagen 4 Polyclonal Antibody, Cy7 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain.
Reactivity Human, Mouse, Rat, Rabbit
Predicted Reactivity Dog, Cow, Pig, Horse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain
Immunogen Range 1401-1500/1669
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 12826
Swiss Prot P02463
Subcellular location Secreted, Cell membrane, Extracellular matrix
Synonyms Bru; Raw; Svc; Col4a-1; Del(8)44H; Collagen alpha-1(IV) chain; Col4a1
Background Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200