Vimentin Polyclonal Antibody, FITC Conjugated

$U2OS cells(black) were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VIM Antibody\uff08bs-0756R-FITC\uff09 at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

U2OS cells(black) were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VIM Antibody\uff08bs-0756R-FITC\uff09 at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Applications

WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Pig
Others
Monkey

Predicted Reactivity

Cow
Chicken
Goat

Overview
Catalog #	bs-0756R-FITC
Product Name	Vimentin Polyclonal Antibody, FITC Conjugated
Applications	WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	KO-Validated
Reactivity	Human, Mouse, Rat, Bovine, Pig, Others, Monkey
Predicted Reactivity	Cow, Chicken, Goat
Specifications
Conjugation	FITC
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Vimentin
Immunogen Range	371-466/466
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID	7431
Swiss Prot	P08670
Subcellular location	Cytoplasm, Extracellular matrix
Synonyms	HEL113; CTRCT3; Vimentin; VIM
Background	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

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