KISS1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded human colon cancer Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human colon cancer Rabbit Anti-Kiss 1\/Kisspeptin Polyclonal Antibody (bs-0749R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kisspeptin Polyclonal Antibody, Unconjugated (bs-0749R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin-embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Kisspeptin Polyclonal Antibody, Unconjugated (bs-0749R) at 1:400 overnight at 4\u00b0C, followed by secondary and DAB staining.

$Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with KISS1 Polyclonal Antibody, Unconjugated (bs-0749R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human SW480 cell lysates; Lane 3: Human DU145 cell lysates probed with KISS1 Polyclonal Antibody, Unconjugated (bs-0749R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep

Overview
Catalog #	bs-0749R
Product Name	KISS1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody detects Kisspeptin-10 region of Kisspeptin.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human KISS1
Immunogen Range	81-145/145
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3814
Swiss Prot	Q15726
Subcellular location	Secreted
Synonyms	HH13; KiSS-1; Metastasis-suppressor KiSS-1; Kisspeptin-1; KISS1; PP598
Background	Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-1 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-1, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-1 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200