B7H4 Polyclonal Antibody

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled Anti-B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human gastric carcinoma labeled Anti-B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human colon carcinoma labeled Anti-B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Hela cells probed with B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Uterus tissue lysates; Lane 4: Rat Kidney tissue lysates ; Lane 5: Human JEG-3 cell lysates ; Lane 6: Human MCF-7 cell lysates probed with B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Rat Uterus tissue lysates; Lane 4: Rat Kidney tissue lysates ; Lane 5: Human JEG-3 cell lysates ; Lane 6: Human MCF-7 cell lysates probed with B7H4 Polyclonal Antibody, Unconjugated (bs-0673R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog

Overview
Catalog #	bs-0673R
Product Name	B7H4 Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Rat B7H4
Immunogen Range	50-100/282
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	295322
Swiss Prot	Q501W4
Subcellular location	Cell membrane
Synonyms	RGD1311204; V-set domain-containing T-cell activation inhibitor 1; Vtcn1
Background	Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation (By similarity).
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200