TGF Beta R1 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain tissue labeled Anti-TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$L1 rat brain lysates L2 mouse embryo lysates probed with Anti TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 55kD. Observed band size:55kD.\\n$

L1 rat brain lysates L2 mouse embryo lysates probed with Anti TGF Beta R1\/TGFBR1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 55kD. Observed band size:55kD.\\n

Lane 1: Mouse Placenta lysates; Lane 2: Rat Small intestine lysates probed with TGF Beta R1 Polyclonal Antibody, Unconjugated (bs-0638R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine

Predicted Reactivity

Overview
Catalog #	bs-0638R
Product Name	TGF Beta R1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine
Predicted Reactivity	Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TGF-beta R1
Immunogen Range	301-400/501
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7046
Swiss Prot	P36897
Subcellular location	Cell membrane
Synonyms	AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; TGF-beta receptor type-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; Serine/threonine-protein kinase receptor R4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I; TGFBR1
Background	Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200