Endostatin Polyclonal Antibody

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Endostatin Polyclonal Antibody (bs-0547R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Liver; Probed with Endostatin Polyclonal Antibody, Unconjugated (bs-0547R) at 1:500, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Liver; Probed with Endostatin Polyclonal Antibody, Unconjugated (bs-0547R) at 1:500, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0547R
Product Name	Endostatin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human COL18A1/Endostatin
Immunogen Range	1581-1680/1754
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	80781
Swiss Prot	P39060
Subcellular location	Secreted, Extracellular matrix
Synonyms	KS; KNO; KNO1; Collagen alpha-1(XVIII) chain; COL18A1
Background	COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200