CD2AP Polyclonal Antibody

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-CD2AP Polyclonal Antibody (bs-0512R) , Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with CD2AP Polyclonal Antibody, Unconjugated (bs-0512R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Rat Kidney tissue lysates probed with CD2AP Polyclonal Antibody, Unconjugated (bs-0512R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse
Rat

Predicted Reactivity

Human
Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig

Overview
Catalog #	bs-0512R
Product Name	CD2AP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse, Rat
Predicted Reactivity	Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD2AP
Immunogen Range	561-639/639
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23607
Swiss Prot	Q9Y5K6
Subcellular location	Cytoplasm
Synonyms	CMS; CD2-associated protein; Adapter protein CMS; Cas ligand with multiple SH3 domains; CD2AP
Background	Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500