beta Actin Polyclonal Antibody

Rat lung lysate 30ug, probed (bs-0061R) at 1:20,000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.

Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.

$A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.$

A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.

Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Lung lysates; Lane 2: Mouse Cerebrum lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Lane 1: Mouse Thymus lysates; Lane 2: Mouse Urinary bladder lysates; Lane 3: Mouse Uterus lysates; Lane 4: Mouse Aorta lysates ; Lane 5: Mouse olfactory bulb lysates; Lane 6: Mouse Cerebellum lysates; Lane 7: Mouse Adrenal gland lysates; Lane 8: Mouse Ovary lysates; Lane 9: Mouse Ear lysates; Lane 10: U2os cell lysates; Lane 11: ASPC-1 cell lysates ; Lane 12: Mouse Vas deferens lysates; Lane 13: Mouse trachea lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: A549 cell lysates; Lane 2: Raw264.7 cell lysates; Lane 3: SH-SY5Y cell lysates; Lane 4: MKN45 cell lysates ; Lane 5: CHO cell lysates; Lane 6: Panc-1 cell lysates; Lane 7: 4T1 cell lysates; Lane 8: ASPC-1 cell lysates; Lane 9: H9C2 cell lysates; Lane 10: Brl-3a cell lysates; Lane 11: TT cell lysates ; Lane 12: TEV-1 cell lysates; Lane 13: EC9706 cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human HL60 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Rat Spinal cord lysates; Lane 4: Rat Rectum lysates ; Lane 5: Rat Placenta lysates; Lane 6: Rat Lymph node lysates; Lane 7: Rat Lung lysates; Lane 8: Rat Spleen lysates; Lane 9: Human JAR cell lysates; Lane 10: Human 293T cell lysates; Lane 11: Human Jurkat cell lysates ; Lane 12: Human TM4 cell lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

NIH\/3T3 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Actin Polyclonal Antibody(bs-0061R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

$Sample:\r\nLymph node (Rat) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/1000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD$

Sample:\r\nLymph node (Rat) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/1000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD

$Sample:\r\nThymus (Mouse) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/1000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD$

Sample:\r\nThymus (Mouse) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/1000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD

$Sample:\r\nSH-SY5Y (Human) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/2000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD$

Sample:\r\nSH-SY5Y (Human) Lysate at 40 ug\r\nPrimary:\r\nAnti-beta-Actin (bs-0061R) at 1\/2000~1\/20000 dilution\r\nSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution\r\nPredicted band size: 42 kD\r\nObserved band size: 42 kD

Applications

WB
ELISA
FCM
IHC-P
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Dog
Pig
Sheep
Rabbit
Goat
Others
All

Overview
Catalog #	bs-0061R
Product Name	beta Actin Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	MAP conjugated synthetic peptide derived from human beta Actin
Immunogen Range	1-200/375
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	60
Swiss Prot	P60709
Subcellular location	Cytoplasm
Synonyms	BRWS1; PS1TP5BP1; Actin, cytoplasmic 1; Beta-actin; ACTB
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Application Dilution
WB	1:5000-50000
ELISA	1:5000-20000
FCM	1:100-500
IHC-P	1:200-400
IF(IHC-P)	1:100-200
IF(IHC-F)	1:50-200
IF(ICC)	1:100