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Bcl-2 Polyclonal Antibody, AbBy Fluor® 750 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Chicken
  • Rabbit
  • Bacteria

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-0032R-BF750
Product Name Bcl-2 Polyclonal Antibody, AbBy Fluor® 750 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat, Bovine, Chicken, Rabbit, Bacteria
Predicted Reactivity Dog, Cow, Pig, Horse
Specifications
Conjugation AbBy Fluor® 750
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat Bcl-2
Immunogen Range 101-160/236
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24224
Swiss Prot P49950
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Bcl-2; Apoptosis regulator Bcl-2; Bcl2
Background Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200