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Primary Antibodies

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Catalog #1: bs-6253R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Cy3

Catalog #1: bs-6253R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: FITC

Catalog #1: bs-6253R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig, Sheep, Others

Conjugate: Cy7

Catalog #1: bs-0920R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-0778R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Others

Conjugate: HRP

Mouse lung lysates probed with TSPAN14 Polyclonal Antibody, Unconjugated (bs-9422R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: mouse uterus lysates; Lane 2: mouse ovary lysates probed with TSPAN14 Polyclonal Antibody, Unconjugated (bs-9422R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Spleen lysates; Lane 4: Mouse Lung lysates probed with TSPAN14 Polyclonal Antibody, Unconjugated (bs-9422R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9422R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-11276R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-6961R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Sheep, Others

Conjugate: Unconjugated

Catalog #1: bs-6961R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Sheep, Others

Conjugate: Biotin

Catalog #1: bs-6961R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat, Sheep, Others

Conjugate: HRP

Catalog #1: bs-6961R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Sheep, Others

Conjugate: Cy3

Catalog #1: bs-6961R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Sheep, Others

Conjugate: Cy5

Catalog #1: bs-6961R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Sheep, Others

Conjugate: Cy7

Catalog #1: bs-6961R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat, Sheep, Others

Conjugate: FITC

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-STK31 Polyclonal Antibody, Unconjugated (bs-7051R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: MCF-7 cell lysates; Lane 2: A431 cell lysates probed with STK31 Polyclonal Antibody, Unconjugated (bs-7015R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse testis lysates; Lane 2: A549 cell lysates probed with STK31 Polyclonal Antibody, Unconjugated (bs-7015R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7015R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-7015R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Biotin

Catalog #1: bs-7015R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: HRP

Catalog #1: bs-7015R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy3

Catalog #1: bs-7015R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5

Catalog #1: bs-7015R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy7

Catalog #1: bs-7015R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-7685R-HRP

Applications: ELISA

Reactivity: Human, Rat, Sheep

Conjugate: HRP

Catalog #1: bs-7685R-Biotin

Applications: ELISA

Reactivity: Human, Rat, Sheep

Conjugate: Biotin

Antigen: bs-7685P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded rat cardiac muscle labeled with Rabbit Anti Corin Polyclonal Antibody, Unconjugated (bs-7685R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Blank control:HepG2. \r\nPrimary Antibody (green line): Rabbit Anti-Corin antibody (bs-7685R) \r\nDilution: 1ug\/Test; \r\nSecondary Antibody : Goat anti-rabbit IgG-AF488\r\nDilution: 0.5ug\/Test. \r\nProtocol\r\nHela cells were\u00a0incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Corin Polyclonal Antibody(bs-7685R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-7685R

Applications: ELISA, IF(ICC)

Reactivity: Human, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-7685R-Cy5

Applications: IF(ICC)

Reactivity: Human, Rat, Sheep

Conjugate: Cy5

Catalog #1: bs-7685R-Cy3

Applications: IF(ICC)

Reactivity: Human, Rat, Sheep

Conjugate: Cy3

Catalog #1: bs-7685R-FITC

Applications: IF(ICC)

Reactivity: Human, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-7685R-Cy7

Applications: IF(ICC)

Reactivity: Human, Rat, Sheep

Conjugate: Cy7

Catalog #1: bs-8600R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat, Pig, Sheep

Conjugate: Unconjugated

Catalog #1: bs-8656R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Sheep

Conjugate: Unconjugated

Rat lung lysate 30ug, probed (bs-0061R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Unconjugated

Catalog #1: bsm-0978M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Biotin

Catalog #1: bsm-0978M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: HRP

Human HepG2 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Human A549 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: mouse placenta; Lane 2: 293T cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0978M

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human U251 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human 293T cell lysates; Lane 5: Human MDA-MB-231 cell lysates probed with GLIS3 Polyclonal Antibody, Unconjugated (bs-16249R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-16249R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-0486R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Others

Conjugate: PE

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4\u00b0C, DAB staining.
Mouse cerebllumlysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-23376R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Unconjugated

Catalog #1: bs-23376R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Biotin

Catalog #1: bs-23376R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: HRP

Catalog #1: bs-23376R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy3

Catalog #1: bs-23376R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy5

Catalog #1: bs-23376R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: Cy7

Catalog #1: bs-23376R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep

Conjugate: FITC

Catalog #1: bs-20782R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Sheep

Conjugate: Biotin

Catalog #1: bs-20782R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Sheep

Conjugate: Unconjugated

Catalog #1: bs-20782R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Sheep

Conjugate: HRP

Catalog #1: bs-12245R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Sheep

Conjugate: Cy5.5

Catalog #1: bs-12880R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Sheep

Conjugate: Cy5.5