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Primary Antibodies



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Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Anti- IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 followed by conjugation to the secondary antibody
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKK_\/_ Polyclonal Antibody(bs-4880R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IKK beta Polyclonal Antibody, Unconjugated (bs-4880R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4880R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4880R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-4880R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-4880R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Rabbit Anti Phospho-IRAK1 (Thr387) Polyclonal Antibody, Unconjugated (bs-3194R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-IRAK1 (Thr387) Polyclonal Antibody, Unconjugated (bs-3194R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with\\tIRAK1 (Thr387) Polyclonal Antibody, Unconjugated (bs-3194R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (Secondary Catalog #) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #1: bs-3194R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4880R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4880R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4880R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-3194R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3194R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3194R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3194R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-3194R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-IRAK1 Polyclonal Antibody, Unconjugated (bs-6464R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IRAK1 Polyclonal Antibody(bs-6464R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (IRAK1) polyclonal Antibody, Unconjugated (bs-6464R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-6464R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3194R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-6464R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-6464R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-6464R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-6464R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-6464R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-6464R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Mouse heart lysates probed with Rabbit Anti-GSK3 alpha\/beta (Tyr279+Tyr216) Polyclonal Antibody, Unconjugated (bs-2073R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
RSC96 cells probed with Rabbit Anti-GSK3 Alpha\/Beta(Tyr279+Tyr216) Polyclonal Antibody (bs-2073R), 3ug,for 20 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:200 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n
Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Pancreas lysates; Lane 5: Mouse Thymus lysates; Lane 6: Rat Muscle lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Cerebrum lysates; Lane 9: Rat Pancreas lysates; Lane 10: Rat Thymus lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human A431 cell lysates; Lane 13: Human U-2OS cell lysates probed with GSK3 Alpha\/Beta Polyclonal Antibody, Unconjugated (bs-2073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2073R

Applications: WB, ELISA, FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2073R-Biotin

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2073R-HRP

Applications: WB, ELISA, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-2073R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-2073R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-2073R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-2073R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-2073R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-phospho-ASK1(Thr845) Polyclonal Antibody, Unconjugated (bs-3031R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat heart, L2 rat brain lysates probed (bs-3031R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 155kDa.
Lane 1: Rat Cerebrum lysates; Lane 2: Rat Stomach lysates probed with phospho-ASK1 Polyclonal Antibody, Unconjugated (bs-3031R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ASK1 (Thr845) Polyclonal Antibody(bs-3031R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3031R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-3031R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #1: bs-3031R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-3031R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-3031R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-3031R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-3031R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3195R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #1: bs-3195R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #1: bs-3195R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: HRP

Catalog #1: bs-3195R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-3195R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-3195R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: FITC

Catalog #1: bs-3195R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy5

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2993R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Unconjugated

Catalog #1: bs-2993R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Biotin

Catalog #1: bs-2993R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: HRP

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Muscle lysates; Lane 2: Mouse Stomach lysates; Lane 3: Rat Muscle lysates probed with IRAK4 Polyclonal Antibody, Unconjugated (bs-2440R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2440R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2993R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Cy3