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Primary Antibodies

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Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-9439P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXO1A Polyclonal Antibody(bs-9439R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Rat Lymph node lysates; Lane 2: Rat Bone lysates; Lane 3: Rat Liver lysates probed with FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9439R

Applications: WB, ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Unconjugated

Catalog #1: bs-9439R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: HRP

Catalog #1: bs-9439R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy3

Catalog #1: bs-9439R-Cy5.5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy5.5

Catalog #1: bs-9439R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: FITC

Catalog #1: bs-9439R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy5

Catalog #1: bs-9439R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Others

Conjugate: Cy7

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human Siha cell lysates probed with PI3KCA Polyclonal Antibody, Unconjugated (bs-5570R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PI3KCA(Tyr317) Polyclonal Antibody, Unconjugated (bs-5570R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-5570R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Unconjugated

Catalog #1: bs-5570R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Bovine

Conjugate: Biotin

Catalog #1: bs-5570R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Bovine

Conjugate: HRP

Catalog #1: bs-5570R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Cy5

Catalog #1: bs-5570R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Cy3

Catalog #1: bs-5570R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Cy5.5

Catalog #1: bs-5570R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: FITC

Catalog #1: bs-5570R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Bovine

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CDX2\/3 Polyclonal Antibody, Unconjugated (bs-9236R) at 1:200 followed by conjugation to the secondary antibody
Lane 1: rat brain lysates Lane 2: rat intestine lysates probed with Anti CDX2\/3 Polyclonal Antibody, Unconjugated (bs-9236R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 34kD. Observed band size: 34kD.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CDX2+3 Polyclonal Antibody, Unconjugated (bs-9236R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nCDX2+3 Polyclonal Antibody, Unconjugated (bs-9236R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9236R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-9236R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-9236R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: HRP

Catalog #1: bs-9236R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Cy3

Catalog #1: bs-9236R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Cy5

Catalog #1: bs-9236R-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Cy5.5

Catalog #1: bs-9236R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: Cy7

Catalog #1: bs-9236R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT2 (4G12) Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SIRT2 (4G12) Monoclonal Antibody, Unconjugated (bsm-52221R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52221R

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Catalog #1: bs-20596R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (bs-20596R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (bs-20596R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (bs-20596R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 Polyclonal Antibody, Unconjugated (bs-20596R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-20596R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-20596R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-20596R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-20596R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-20596R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-20596R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-20596R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-20596R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-22318R

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-22318R-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-22318R-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-8711R-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep, Rabbit, Horse

Conjugate: Biotin

Catalog #1: bs-8711R

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep, Rabbit, Horse

Conjugate: Unconjugated

Catalog #1: bs-8711R-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Pig, Sheep, Rabbit, Horse

Conjugate: HRP

Catalog #1: bsm-33201M

Applications: WB

Reactivity: Bovine

Conjugate: Unconjugated

Catalog #1: bsm-33201M-Biotin

Applications: WB

Reactivity: Bovine

Conjugate: Biotin

Catalog #1: bsm-33201M-HRP

Applications: WB

Reactivity: Bovine

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with phospho-IRS1 (Tyr632) Polyclonal Antibody, Unconjugated (bs-8707R) at 1:200 overnight at 4\u00b0C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IRS1 (Tyr632) ) polyclonal Antibody, Unconjugated (bs-8707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-8707R

Applications: FCM, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-8707R-Biotin

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-8707R-HRP

Applications: IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: HRP

Catalog #1: bs-8707R-Cy3

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy3

Catalog #1: bs-8707R-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5

Catalog #1: bs-8707R-Cy5.5

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit

Conjugate: Cy5.5