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Primary Antibodies



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Catalog #1: bs-3009R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Raw264.7 cell lysates probed with Bim(Ser87) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3012R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti phospho-B-Raf (Ser365) Polyclonal Antibody, Unconjugated (bs-3013R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with B-Raf (Ser365) Polyclonal Antibody, Unconjugated (bs-3013R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with B-Raf (Ser365) Polyclonal Antibody, Unconjugated (bs-3013R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-B-Raf (Ser365)) Polyclonal Antibody, Unconjugated (bs-3013R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3013R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3015R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

HL-60 cells were fixed with 4% PFA for 10min at room temperature\uff0c0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRCA1 (Ser1524) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Control cells (dark blue), secondary only (light blue), Isotype control (orange) and BRCA1 (Ser1524) (green).
HL-60 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nBRCA1 (Ser1524) Polyclonal Antibody(bs-3014R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3014R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Antigen: bs-3022P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ALK (Tyr1604)) Polyclonal Antibody, Unconjugated (bs-3022R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3022R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-3023R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3026R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-3027P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Formalin-fixed and paraffin embedded human kidney labeled with Anti-phospho-AMPK beta 1 (Ser182) Polyclonal Antibody, Unconjugated (bs-3027R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AMPK beta 1 (Ser182) Polyclonal Antibody(bs-3027R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AMPK beta 1 (Ser182) Polyclonal Antibody, Unconjugated (bs-3027R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3027R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-3028R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3029R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3034R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-3035R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3037R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-Acetyl CoA Carboxylase(Ser79)\/ACACA Polyclonal Antibody, Unconjugated (bs-3039R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer normal goat serum at 37\u00b0C for 30min; Antibody incubation with P-ACACA(Ser79) Polyclonal Antibody, Unconjugated bs-3039R at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bs-3039R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3045R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3047R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3048R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-3049R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3051R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti Phospho-Bcl-2(Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen lysates probed with Anti-Bcl-2 (Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Image submitted by OWL Validation Program. 20 \u03bcg proteins of cell lysates from A431, U266 and THP-1 were separated by SDS-PAGE and transferred to PVDF membrane for western blotting analysis antibodies against bs-3052r Rabbit Anti-Bcl-2 (Ser70) Polyclonal Antibody at dilution of 1:1000 with exposure time of 10 min.
Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Bcl-2 (Ser70) Polyclonal Antibody, Unconjugated (bs-3052R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3052R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3054R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-Btk (Ser180) Polyclonal Antibody (bs-3055R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RAW264.7 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-Btk (Ser180) Polyclonal Antibody(bs-3055R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-3055R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (bs-1868R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (bs-3060R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-CEBP alpha (Ser21)) polyclonal Antibody, Unconjugated (bs-3060R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3060R

Applications: ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3064R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3065R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3066R

Applications: WB, ELISA, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3067R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-BTK (Tyr223) Polyclonal Antibody, Unconjugated (bs-3069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3069R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3071R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3072R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Hela cell lysates; Lane 2: 293T cell lysates probed with HDAC4(Ser246)\/HDAC5(Ser259)\/HDAC7(Ser155) Polyclonal Antibody, Unconjugated (bs-3073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3073R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was used at a 100 fold (A and B) and 500 fold (C and D) dilution. A no primary antibody negative control (E and F) was used to verify the specificity of the stain. The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. \r\nImages in the upper row (A, C, and E) were captured at 10x magnification, while the images below (B, D, and F) were taken at 20x magnification. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program. \r\n
Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MCSF Receptor (Tyr723) Polyclonal Antibody(bs-3077R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3077R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-MCSF Receptor(Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Hela cell lysates probed with MCSF Receptor (Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raw264.7 cell lysates probed with MCSF Receptor (Tyr923) Polyclonal Antibody, Unconjugated (bs-3079R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
MCF-7 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CSF1R (Tyr923) Antibody\uff08bs-3079R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-3079R

Applications: WB, ELISA, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-3080P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm.
Lane 1: rat brain lysates Lane 2: human gastric carcinoma lysates probed with Anti phospho-CYLD(Ser418)Polyclonal Antibody, Unconjugated (bs-3080R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 105kD. Observed band size: 105kD.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CYLD (Ser418) Polyclonal Antibody, Unconjugated (bs-3080R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3080R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-phospho-CaMK2 alpha (Tyr231)Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CaMK2 (Tyr230) Polyclonal Antibody(bs-3081R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (bs-3081R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3081R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Beta-Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
293T cell lysates probed with Beta-Catenin (Ser33 + Ser37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with beta Catenin (Ser33 + 37) Polyclonal Antibody, Unconjugated (bs-3084R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3084R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3085R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3087R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-3089R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3090R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Antigen: bs-3091P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-CDK1 (Thr14)) polyclonal Antibody, Unconjugated (bs-3091R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-CDK1 (Thr14) Polyclonal Antibody(bs-3091R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Lymph node tissue lysates; Lane 3: Mouse Thymus tissue lysates; Lane 4: Rat Thymus tissue lysates ; Lane 5: Human SH-SY5Y cell lysates probed with Phospho-CDK1 (Thr14) Polyclonal Antibody, Unconjugated (bs-3091R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3091R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Anti Phospho-cdc2 (Tyr15) Polyclonal Antibody, Unconjugated (bs-3092R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cell lysates probed with Phospho-CDK1 (Tyr15) Polyclonal Antibody, Unconjugated (bs-3092R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3092R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3093R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Mouse Embryo lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2: Human MCF-7 lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-cdc25A (Ser76) Antibody(bs-3094R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-3094R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (cdc25A (Ser178)) Polyclonal Antibody, Unconjugated (bs-3095R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (cdc25A (Ser178)) Polyclonal Antibody, Unconjugated (bs-3095R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3095R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Phospho-cdc25C(Ser216)Polyclonal Antibody, Unconjugated (bs-3096R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse testis lysates probed with Rabbit Anti-cdc25C (Ser216) Polyclonal Antibody, Unconjugated (bs-3096R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: Mouse Embryo lysates probed with cdc25C (Ser216) Polyclonal Antibody, Unconjugated (bs-3096R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3096R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse embryo lysates probed with Anti Phospho-CDK9 (Thr186) Polyclonal Antibody, Unconjugated (bs-3097R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 43kD. Observed band size: 43kD.
Lane 1: Mouse Brain lysates probed with CDK9 (Thr186) Polyclonal Antibody, Unconjugated (bs-3097R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3097R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated