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Primary Antibodies



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Catalog #1: bs-2089R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2086R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Bacteria

Conjugate: Unconjugated

Catalog #1: bs-2084R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
MCF7 cells were fixed with 70% ice-cold methanol overnight at 4\u2103, permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with P53(wt-p53) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at -20\u2103, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.
Lane 1: Mouse Liver lysates; Lane 2: Rat Liver lysates probed with P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Tissue\/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (P53(wt-p53)) polyclonal Antibody, Unconjugated (bs-2090R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2090R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Goat

Conjugate: Unconjugated

Catalog #1: bs-2093R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Mouse, Pig

Conjugate: Unconjugated

Human U937 cells probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Human Hela cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human U-87MG cell lysates; Lane 4: Human HepG2 cell lysates; Lane 5: Human A549 cell lysates probed with Integrin alpha 1 Polyclonal Antibody, Unconjugated (bs-2095R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2095R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-SLC4A4 Polyclonal Antibody, Unconjugated(bs-2096R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Kidney lysates probed with kNBC Polyclonal Antibody, Unconjugated (bs-2096R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2096R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiotensin 2 Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-2097R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiotensin 2 Type 1 Receptor) Polyclonal Antibody, Unconjugated (bs-2097R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2097R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti Estrogen Receptor alpha Polyclonal Antibody (bs-2098R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Estrogen Receptor alpha Polyclonal Antibody(bs-2098R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha Polyclonal Antibody, Unconjugated (bs-2098R) at 1:200 overnight at 4\u00b0C, DAB staining.
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-2098R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2098R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10994R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-TNFAIP1 Polyclonal Antibody, Unconjugated (bs-2104R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TNFAIP1 Polyclonal Antibody, Unconjugated (bs-2104R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2104R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-2109P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: E.coli Lysate lysates probed with V5 tag Polyclonal Antibody, Unconjugated (bs-2109R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: V5-Tagged Fusion Protein Overexpression E.coli Lysate (bs- 41230P) probed with V5 tag Polyclonal Antibody, Unconjugated (bs-2109R) at 1:100000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2109R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Antigen: bs-2110P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: E.coli Lysate lysates probed with VSV-G tag Polyclonal Antibody, Unconjugated (bs-2110R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2110R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-2112R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Tags

Conjugate: Unconjugated

Catalog #1: bs-2113R

Applications: ELISA, ICC

Reactivity: Tags

Conjugate: Unconjugated

Catalog #1: bs-2116R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-2115R

Applications: WB, ELISA, ICC

Reactivity: Tags, Others

Conjugate: Unconjugated

Catalog #1: bs-2121R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Lane 1: GST-Tagged Fusion Protein Overexpression E.coli lysates; Lane 2: GST-Tagged Fusion Protein Overexpression E.coli lysates probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: GST-tagged fusion protein probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4\u2103 overnight.

Catalog #1: bs-2122R

Applications: WB, ELISA

Reactivity: Tags, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human stomach labeled with Anti-RNF126 Polyclonal Antibody, Unconjugated (bs-2125R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse liver lysates probed with RNF126 Polyclonal Antibody, Unconjugated (bs-2125R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2125R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

L1 rat brain, L2 rat heart lysates probed (bs-2127R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 43kDa.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 5HT4 Receptor Polyclonal Antibody, Unconjugated (bs-2127R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 5HT4 Receptor Polyclonal Antibody (bs-2127R) at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) by operating according instructions and DAB staining.
Lane 1: Mouse Stomach tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Adrenal gland tissue lysates ; Lane 5: Rat Adrenal gland tissue lysates probed with 5HT4 Receptor Polyclonal Antibody, Unconjugated (bs-2127R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2127R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti- 5-HTR3Polyclonal Antibody, Unconjugated(bs-2126R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebellum lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Mouse Spinal cord lysates; Lane 4: Mouse Heart lysates ; Lane 5: Rat Heart lysates probed with 5-HTR3\/HTR3A Polyclonal Antibody, Unconjugated (bs-2126R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2126R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-2128R

Applications: WB, ELISA, IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Mouse kidney lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Placenta lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Embryo lysates; Lane 6: Mouse Cerebrum lysates; Lane 7: Mouse NIH\/3T3 cell lysates; Lane 8: Rat Liver lysates; Lane 9: Rat Heart lysates; Lane 10: Rat Kidney lysates; Lane 11: Rat Cerebrum lysates; Lane 12: Rat Embryo lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (bs-2132R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-2132R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2133R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2135R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysates probed with Rabbit Anti-Thrombin light chain Polyclonal Antibody, Unconjugated (bs-2134R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse placenta lysates probed with Rabbit Anti-Thrombin light chain Polyclonal Antibody, Unconjugated (bs-2134R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2134R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2138R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-2139R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-IL-17 Polyclonal Antibody, Unconjugated(bs-2140R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: MOLT-4 cell lysates; Lane 2: U937 cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raji cell lysates probed with IL17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates probed with IL-17 Polyclonal Antibody, Unconjugated (bs-2140R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2140R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2143R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-2147R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human ovarian cancer labeled with Anti-HER2 receptor(NT) Polyclonal Antibody, Unconjugated(bs-2156R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Recombinant human HER2 protein, N-His(bs-42263P) probed with HER2 receptor(NT) Polyclonal Antibody, Unconjugated (bs-2156R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2156R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2149R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2157R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2158R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-2159R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2160R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-DKK1 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-DKK1 Polyclonal Antibody, Unconjugated (bs-2162R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2162R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-ADAR1 (N-terminus) Polyclonal Antibody, Unconjugated(bs-2167R) followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human A549 cell lysates; Lane 2: Human U251 cell lysates probed with ADAR1 Polyclonal Antibody, Unconjugated (bs-2167R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-2167R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2168R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2169R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2170R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2171R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2172R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon labeled with Anti-OLFM1 Polyclonal Antibody, Unconjugated (bs-2173R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti OLFM1\/Noelin Polyclonal Antibody, Unconjugated (bs-2173R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 51kD. Observed band size: 35kD, 51kD.

Catalog #1: bs-2173R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-2176R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-2175R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated