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Primary Antibodies

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HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
NIH\/3T3 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with c-Jun (4A11) Monoclonal Antibody (bsm-52035R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52035R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52204R

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Human heart; Lane 2: Human skeletal muscle lysate probed with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
NIH\/3T3 cells were stained with Hsp27(S78) (5E1 ) Monoclonal Antibody (bsm-52167R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa + Anisomycin(-), 2 HeLa+ Anisomycin(+) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 23 kDa Observed MW: 27 kDa
Tissue: Human breast cancer Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:500 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52167R\r\n

Catalog #1: bsm-52167R

Applications: WB, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of SH-Sy5Y cells with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Rat brain; Lane 2: Mouse brain; Probed with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C
IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Prion Protein(PrP) (33A5) Monoclonal Antibody (bsm-52505R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52505R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7 Cells; Lane 2: THP-1 Cells; Lane 3: HepG2 Cells; Probed with NOX2\/gp91phox (9B12) Monoclonal Antibody (bsm-52603R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates probed with NOX2\/gp91phox Monoclonal Antibody, Unconjugated (bsm-52603R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-52603R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: THP-1 Cells; Lane 2: Raji Cells; Probed with CD38 (10A10) Monoclonal Antibody (bsm-52623R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD38 (10A10) Monoclonal Antibody (bsm-52623R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD38 (10A10) Monoclonal Antibody (bsm-52623R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD38 (10A10) Monoclonal Antibody, Unconjugated (bsm-52623R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52623R

Applications: WB, IHC

Reactivity: Human

Conjugate: Unconjugated

293 cells were stained with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: COS-7, 3: K562 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 131 kDa Observed MW: 160 kDa
Tissue: Human kidney Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52086R\r\n

Catalog #1: bsm-52086R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-34235R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10927R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-19351R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-19774R

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-13768R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14367R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21613R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21783R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23716R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33423M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-16731R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Siha cell lysates probed with ARL13B Polyclonal Antibody, Unconjugated (bs-21493R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with ARL13B Polyclonal Antibody, Unconjugated (bs-21493R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-21493R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7462R

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-17709R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7499R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10940R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-20457R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-10837M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-10837M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-10837M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Ki-67) Monoclonal Antibody, Unconjugated (bsm-10837M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-10837M

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Beta catenin) Monoclonal Antibody, Unconjugated (bsm-33071M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33071M

Applications: IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33226M

Applications: WB

Reactivity: Arabidopsis Thaliana

Conjugate: Unconjugated

Catalog #1: bs-23671R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-41176R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10879M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41199R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-22310R

Applications: IHC-P

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-41233R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25072R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-49189R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43017M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41388R

Applications: WB

Reactivity: JEV

Conjugate: Unconjugated

Catalog #1: bs-41337R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25355R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-25366R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-41330M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43025M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-41334R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-42049M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Recombinant human C-jun protein(50ng) probed with C-jun Monoclonal Antibody, Unconjugated (bsm-42050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (C-jun) polyclonal Antibody, Unconjugated (bsm-42050M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-42050M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-42025R

Applications: WB

Reactivity: Cow

Conjugate: Unconjugated

Catalog #1: bsm-43518M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-42005M

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated