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Primary Antibodies

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Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): HeLa, (+): HeLa+ sodium\r\nbutyrate (30mM, 4hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14kDa Observed MW: 14kDa
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium\r\nbutyrate (50mM, 24hr) + trichostain A (500ng\/ml, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14kDa Observed MW: 14kDa

Catalog #1: bsm-60644R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60656R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium\r\nbutyrate (50mM, 24hr) + trichostain A (500ng\/ml, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 4\u2103 overnight Secondary Ab: (-): NIH\/3T3, (+): NIH\/3T3+ trichostain A (1\u03bcM, 18 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 14 KDa Observed MW: 14 KDa

Catalog #1: bsm-60663R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room\r\ntemperature Secondary Ab:1: HeLa cytosol, 2: HeLa nuclear Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room\r\ntemperature Secondary Ab:1: Mouse brain, 2: Rat brain, 3: Recombinant histone H3 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 KDa Observed MW: 15 KDa

Catalog #1: bsm-60666R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): MCF-7+serum starvation (14 hr), (+): MCF-7+ sodium butyrate (50 mM, 24 hr) +\r\ntrichostain A (500 ng\/ml, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+ sodium butyrate\r\n(30 mM, 4 hr) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa
Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:2000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Neuro-2a, 2: BRL Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 15 kDa Observed MW: 15 kDa

Catalog #1: bsm-60689R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary Ab dilution: 1:1000\r\nPrimary Ab incubation condition: 2 hours at \r\nroom temperature\r\nSecondary Ab: Goat Anti-Rabbit IgG H&L \r\n(HRP)\r\nLysate: (-): MCF-7+serum starvation(14hr), (+): \r\nMCF-7+ sodium butyrate (50mM, 24hr) + \r\ntrichostain A (500ng\/ml, 4 hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 50 kDa\r\nObserved MW: 55 kDa
Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60706R\r\n
Tissue: Rat cerebellum\r\nSection type: Formalin-fixed & Paraffin\u0002embedded section \r\nRetrieval method: High temperature and high \r\npressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 \r\nPrimary Ab dilution: 1:100\r\nPrimary Ab incubation condition: 1 hour at \r\nroom temperature \r\nSecondary Ab: Anti-Rabbit and Mouse \r\nPolymer HRP (Ready to use)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal \r\nfor bsm-60706R\r\n

Catalog #1: bsm-60706R

Applications: WB, IHC-P, IHC-F, IF, ICC

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60750R

Applications: WB

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-16147R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-22138R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-23454R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-10674R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-25034R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-25032R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41439R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-23630R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-43520R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with alpha Internexin Polyclonal Antibody, Unconjugated (bs-42003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human SH-SY5Y cell lysates probed with alpha Internexin Polyclonal Antibody, Unconjugated (bs-42003R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-42003R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41378R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-25183R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-24294R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-24293R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41304R

Applications: WB

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-41361R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-16732R

Applications: WB

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-43079M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43099M

Applications: WB

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-43007M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Recombinant human Beta-2-microglobulin, C-His (HEK293) probed with beta 2 Microglobulin Monoclonal Antibody, Unconjugated (bsm-43094M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Raji cell lysates; Lane 5: Human PANC-1 cell lysates probed with beta 2 Microglobulin Monoclonal Antibody, Unconjugated (bsm-43094M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-43094M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-43005M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41611M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41482M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41483M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41512M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41513M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Lane 1: Human Raji cell lysates probed with CD20 Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD20) Monoclonal Antibody, Unconjugated (bsm-60638R) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60638R

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-34219M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-41503M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41504M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41517M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41518M

Applications: WB

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bs-10772R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-14993R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat lung lysates probed with Rabbit Anti-GAD65 Polyclonal Antibody, Unconjugated (bs-0325R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Hippocampus tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Hippocampus tissue lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Human U-87 MG cell lysates; Lane 9: Human Hela cell lysates; Lane 10: Human Jurkat cell lysates probed with GAD65 Polyclonal Antibody, Unconjugated (bs-0325R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0325R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7369R

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-21404R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562; Lane 2: Hela lysate probed with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
PC-3 cells were stained with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with STAT5b (12A1) Monoclonal Antibody (bsm-52238R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52238R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: MCF-7 cell lysates; probed with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
A431 cells were stained with 14-3-3 Theta (5G1) Monoclonal Antibody (bsm-52006R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Catalog #1: bsm-52006R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Jurkat; Lane 2: K562; Lane 3: Hela lysates probed with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .
SW480 cells were stained with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37\u00b0C for 20min; Antibody incubation with Cdk6 (4F7) Monoclonal Antibody (bsm-52030R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52030R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated