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Primary Antibodies

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Catalog #1: bs-0397R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-0397R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse brain lysates L2 mouse heart lysates probed with Anti MMP9 Polyclonal Antibody, Unconjugated (bs-4593R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 78kD. Observed band size: 78kD.

Catalog #1: bs-4593R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-4593R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: Biotin

Catalog #1: bs-4593R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Conjugate: HRP

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-P-selectin Polyclonal Antibody (bs-0561R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti-CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Lane 1: K562 cell lysates; Lane 2: Huvec cell lysates; Lane 3: Jurkat cell lysates probed with CD62p Polyclonal Antibody, Unconjugated (bs-0561R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0561R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0561R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Biotin

Catalog #1: bs-0561R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Spleen lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Hela lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1287R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Unconjugated

Catalog #1: bs-1287R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: Biotin

Catalog #1: bs-1287R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken

Conjugate: HRP

Catalog #1: bs-3589R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bs-3589R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Lane 1: Rat brain lysates; Lane 2: Rat liver lysates probed with Rabbit Anti-eNOS (Thr113) Polyclonal Antibody (bs-3589R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with eNOS (Thr113) Polyclonal Antibody(bs-3589R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3589R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0920R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Biotin

Catalog #1: bs-0920R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BNP Polyclonal Antibody, Unconjugated (bs-2207R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-BNP Polyclonal Antibody, Unconjugated(bs-2207R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-2207R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-2207R-HRP

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-2207R-Biotin

Applications: ELISA, IHC-P

Reactivity: Rat

Conjugate: Biotin

Lane 1:Mouse kidney lysate Lane 2: Mouse brain lysate probed with Rabbit Anti-Bad Polyclonal Antibody, Unconjugated (bs-1304R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Image provided by the Independent Validation Program (badge number 29802). Lane 1: MCF7, Lane 2: c6\/36 mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-BAD Polyclonal Antibody, Unconjugated (bs-1304R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 26˚C. \\n
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bad ) Polyclonal Antibody, Unconjugated (bs-1304R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Bad ) Polyclonal Antibody, Unconjugated (bs-1304R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1304R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-5768R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2\/N-cadherin Polyclonal Antibody, Unconjugated(bs-1172R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (bs-1172R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-1172R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Unconjugated

Catalog #1: bs-1172R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: HRP

Catalog #1: bs-1172R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Zebrafish, Cow

Conjugate: Biotin

Catalog #1: bs-1629R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat hepatoma labeled with Anti-CXCR2\/CD182 Polyclonal Antibody, Unconjugated (bs-1629R) followed by conjugation to the secondary antibody and DAB staining
Mouse bone lysates probed with Rabbit Anti-CXCR2\/CD182 Polyclonal Antibody, Unconjugated (bs-1629R) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-1629R

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1629R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3101R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-3101R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-3101R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IFN-gamma Polyclonal Antibody, Unconjugated (bs-0480R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Diseased mouse lung and spleen lysates probed with Anti-IFN gamma Polyclonal Antibody (bs-0480R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Lane 1: Mouse Raw264.7-PMA Lysates; Lane 2: Mouse Lymph Lysates; Lane 3: Mouse Spleen Lysates; Lane 4: Mouse Thymus Lysates; Lane 5: Rat Lymph Lysates; Lane 6: Rat Thymus Lysates; Lane 7: Human Jurkat-TPA cell Lysates; Lane 8: Human Jurkat cell Lysates; Lane 9: Human HepG2 cell Lysates; Lane 10: Recombinant human IFN gamma Protein (100ng) (bs-0388P). Probed with IFN gamma polyclonal Antibody, unconjugated (bs-0480R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0480R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0480R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0480R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: mouse brain lysates Lane 2: mouse heart lysates probed with Anti-phospho-BAD(Ser128) Polyclonal Antibody, Unconjugated (bs-0893R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 18kD. Observed band size: 18kD
Formalin-fixed and paraffin embedded rat kidney labeled with Anti-phospho-BAD (Ser128) Polyclonal Antibody, Unconjugated (bs-0893R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded (human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-BAD (Ser128)) Polyclonal Antibody, Unconjugated (bs-0893R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (phospho-BAD (Ser128)) Polyclonal Antibody, Unconjugated (bs-0893R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0893R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0893R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0893R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-3720R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-3018R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Antigen: bs-3018P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Lane 1: mouse kidney lysates Lane 2: mouse embryo lysates probed with Anti phospho-eIF4EBP1\/4EBP1(Ser64) Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 13kD. Observed band size: 13kD
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-eIF4EBP1(Ser64)Polyclonal Antibody, Unconjugated (bs-3018R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-3018R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-3018R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-5334R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5334R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-5334R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-5337R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-5337R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-5337R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti- ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Kamlesh Gupta as part of the free sample program. Caco-2 cell lysate and mouse kidney lysate probed with ZO-1 primary antibody (bs-1329R) at 1:500 overnight at 4˚C. Followed by incubation with anti-rabbit HRP-conjugated secondary antibody at 1:5000 dilution for 1h at 25˚C. Predicted and observed band size: ~200kDa.
Image provided by the Independent Validation Program, badge number 029577:Formalin-fixed and paraffin embedded human testis labeled with Anti-ZO-1 Polyclonal Antibody, Unconjugated (bs-1329R) at 1:250 followed by conjugation to the secondary antibody.
293T (Positive) and HL-60 (Negative control) cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ZO-1 Antibody( bs-1329R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1329R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

Catalog #1: bs-1329R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: HRP