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Primary Antibodies

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Catalog #1: bs-13999R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14026R

Applications: FCM, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10043R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bs-10043R-HRP

Applications: IHC-P, IHC-F

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bs-10050R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bs-10050R-HRP

Applications: IHC-P, IHC-F

Reactivity: Virus

Conjugate: HRP

Catalog #1: bs-14473R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-14473R-Biotin

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-14473R-HRP

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-iNOS Polyclonal Antibody (bs-0162R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat lung lysates Lane 2: rat brain lysates probed with Anti NOS-2\/iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 130kD. Observed band size: 115,130kD.
Lane 1: Mouse Raw264.7 tissue lysates; Lane 2: Human Jurkat cell lysates probed with iNOS Polyclonal Antibody, Unconjugated (bs-0162R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Raw264.7 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a00.1%PBST for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with iNOS Antibody(bs-0162R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0162R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Dog, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat skin tissue labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Anti-Collagen V Polyclonal Antibody, Unconjugated (bs-0552R) 1:200 followed by conjugation to the secondary PE antibody and DAB staining
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Skin tissue lysates; Lane 3: Mouse Uterus tissue lysates; Lane 4: Rat Uterus tissue lysates; Lane 5: Human A549 cell lysates probed with COL5A1 Polyclonal Antibody, Unconjugated (bs-0552R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0552R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-ABCG2\/CD338 Polyclonal Antibody, Unconjugated(bs-0662R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
L1 rat brain lysates L2 mouse embryo lysates probed with Anti ABCG2\/CD338 Polyclonal Antibody, Unconjugated (bs-0662R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 72kD. Observed band size:72kD, 28kD.\\n

Catalog #1: bs-0662R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Nm23) Polyclonal Antibody, Unconjugated (bs-0688R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0688R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Antigen: bs-0719P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-CEA Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CEAM5 Antibody(bs-0719R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEAM5 Polyclonal Antibody, Unconjugated (bs-0719R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0719R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti phospho-Androgen Receptor(Ser578) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (mouse prostate); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Androgen Receptor(Ser578)) Polyclonal Antibody, Unconjugated (bs-0943R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0943R

Applications: IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NET1 Polyclonal Antibody, Unconjugated (bs-10186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Cerebellum tissue lysates; Lane 6: Rat Stomach tissue lysates; Lane 7: Human K562 cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human HepG2 cell lysates; Lane 10: Human MCF-7 cell lysates probed with NET1 Polyclonal Antibody, Unconjugated (bs-10186R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10186R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BRCA1 (Ser1466) Polyclonal Antibody, Unconjugated (bs-12887R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PDZK4 Polyclonal Antibody, Unconjugated (bs-12887R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (bs-12887R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-BRCA1 (Ser1466)) Polyclonal Antibody, Unconjugated (bs-12887R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-12887R

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12887R-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-12887R-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded human smooth muscle labeled with Anti-KLF6\/PAC1 Polyclonal Antibody, Unconjugated (bs-1395R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Human HepG2 cell lysates probed with KLF6 Polyclonal Antibody, Unconjugated (bs-1395R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1395R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1395R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1395R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse colon labeled with Anti-Jagged1\/CD339 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (bs-1448R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Recombinant human CD339 protein, N-His(bs-42225P) probed with Jagged1 Polyclonal Antibody, Unconjugated (bs-1448R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1448R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1448R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1448R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat tongue labeled with Anti-COX4\/COX IV-1 Polyoclonal Antibody, Unconjugated (bs-1533R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human A431 cell lysates probed with COX4I1 Polyclonal Antibody, Unconjugated (bs-1533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1533R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with GPR92 Polyclonal Antibody, Unconjugated (bs-15366R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human K562 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human Jurkat cell lysates probed with GPR92 Polyclonal Antibody, Unconjugated (bs-15366R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-15366R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-15366R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-15366R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1565R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1565R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1565R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti- CCL11\/Eotaxin 1 Polyclonal Antibody (bs-1601R) at 1:100 for two hours at room temperature (green channel).
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CCL11\/Eotaxin 1 ) Polyclonal Antibody, Unconjugated (bs-1601R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1601R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1601R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1601R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Rabbit Anti PI 3 Kinase p85 beta Polyclonal Antibody, Unconjugated (bs-4160R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human colon carcinoma lysates probed with Anti PI 3 Kinase p85 beta Polyclonal Antibody, Unconjugated (bs-4160R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 82kD. Observed band size:82kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PI 3 Kinase p85 beta Polyclonal Antibody, Unconjugated (bs-4160R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PI 3 Kinase p85 beta Polyclonal Antibody, Unconjugated (bs-4160R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-4160R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Catalog #1: bs-4160R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Biotin

Catalog #1: bs-4160R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human pancreas ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (GSK3 Beta (Tyr216) ) Polyclonal Antibody, Unconjugated (bs-4079R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Mouse NIH\/3T3 cell lysates; Lane 4: Rat Cerebrum tissue lysates; Lane 5: Rat Testis tissue lysates probed with phospho-GSK3 Beta (Tyr216) Polyclonal Antibody, Unconjugated (bs-4079R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4079R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #1: bs-4079R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #1: bs-4079R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tAPPL1 Polyclonal Antibody, Unconjugated (bs-1632R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded (human pancreatic); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (APPL1) Polyclonal Antibody, Unconjugated (bs-1632R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (APPL1) Polyclonal Antibody, Unconjugated (bs-1632R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (APPL1) Polyclonal Antibody, Unconjugated (bs-1632R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1632R

Applications: IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CHK1\/CHEK1 Polyclonal Antibody, Unconjugated (bs-1681R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 52kD. Observed band size: 33kD, 52kD.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CHK1\/CHEK1 Polyclonal Antibody, Unconjugated (bs-1681R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CHK1\/CHEK1 Polyclonal Antibody, Unconjugated (bs-1681R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Testis tissue lysates; Lane 3: Rat Testis tissue lysates; Lane 4: Human A431 cell lysates; Lane 5: Human HeLa cell lysates; Lane 6: Human MCF-7 cell lysates probed with CHK1 Polyclonal Antibody, Unconjugated (bs-1681R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1681R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1681R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1681R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti alpha Actinin 4 Polyclonal Antibody, Unconjugated (bs-1741R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 105kD. Observed band size: 105kD.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (alpha Actinin 4) polyclonal Antibody, Unconjugated (bs-1741R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (alpha Actinin 4) polyclonal Antibody, Unconjugated (bs-1741R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Lane 1: Mouse Uterus tissue lysates; Lane 2: Rat Uterus tissue lysates probed with alpha Actinin 4 Polyclonal Antibody, Unconjugated (bs-1741R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1741R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1741R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1741R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP