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Primary Antibodies



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Catalog #1: bsm-30096M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30096M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30096M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30096M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30097M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30097M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30097M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (CD34) polyclonal Antibody, Unconjugated (bsm-30097M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (human CD34) Monoclonal Antibody, Unconjugated (bsm-30097M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CD34 ) Monoclonal Antibody, Unconjugated (bsm-30097M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-30097M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30097M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30097M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30097M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30098M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30098M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30098M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30098M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30098M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30098M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30098M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30099M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30099M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30099M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30099M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30099M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30099M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30099M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30396M

Applications: FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-2652R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-2575R-PE

Applications: WB, FCM

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-9519R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-60548M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-60546R

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60500M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60475M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60414M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60412M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60408M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60395M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60392M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60411M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60410M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60407M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60399M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60272M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60283M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60625R

Applications: WB, FCM, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: MCF-7, 3: HepG2, 4: C6, 5: NIH-3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 29 kDa
Tissue: Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60629R
Tissue: Mouse small intestine\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60629R
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60629R

Catalog #1: bsm-60629R

Applications: WB, FCM, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: NIH-3T3, 3: BRL, 4: Mouse brain, 5: Recombinant histone H3 (20ng)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 15 kDa\r\nObserved MW: 15 kDa
IP of HeLa cells extracts\r\nIP ab incubation condition: bsm-60208R, 4\u00b0C overnight, 1:50 dilution\r\nWB primary ab incubation condition: bsm-60208R, room temperature 2h, 1:2000 dilution\r\nSecondary ab: Anti-Rabbit IgG for IP (HRP)\r\nBlocking buffer and concentration: 5% NFDM\/TBST\r\nDiluting buffer and concentration: 5% NFDM\/TBST\r\n1: 5% Input\r\n2: IP with Rabbit monoclonal IgG Isotype\r\n3: IP with bsm-60208R\r\nObserved MW: 15 kDa\r\nExposure time: 30 s
Tissue: Human breast\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab:SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60208R
Tissue: Mouse cerebrum\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab:SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60208R

Catalog #1: bsm-60208R

Applications: WB, FCM, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1. HeLa, 2. NIH-3T3, 3. BRL, 4. Mouse heart\uff0c5. Rat heart, 6. Mouse kidney, 7. Rat kidney, 8. Recombinant Histone H4\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 11 kDa\r\nObserved MW: 11 kDa
Tissue: Rat stomach\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60152R
Tissue: Mouse liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60152R
Tissue: Human prostate hyperplasis\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary ab dilution: 1:500\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60152R

Catalog #1: bsm-60152R

Applications: WB, FCM, IHC-P, ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated