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Catalog #1: bs-6970R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin-embedded mouse intestine tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with RNA polymerase 2 Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNA polymerase II) Polyclonal Antibody, Unconjugated (bs-6972R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-6972R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-6972R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-6972R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-6972R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-6972R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-7004R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-7004R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-7004R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-7004R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-7004R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti EphA7\/Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Rat brain lysates probed with Rabbit Anti-Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 107kD. Observed band size: 45kD
Lane 1: Mouse Hippocampus cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates probed with Eph receptor A7 Polyclonal Antibody, Unconjugated (bs-7034R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7034R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7034R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-7034R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-7034R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-7034R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NGFR\/p75NTR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse spleen cells were probed with NGFR Polyclonal Antibody (bs-7122R) for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\r\n
Lane 1: Mouse Cerebellum; Probed with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:300, overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Rat Liver tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with NGFR Polyclonal Antibody, Unconjugated (bs-7122R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-7122R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7338R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7338R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-7338R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-7338R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-7338R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

U937 cell lysates probed with Rabbit Anti-CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Staining of Placental villus from 7w gestation karyotype-normal pregnancy with bs-7351R. The Positive Control used is Placental villus from 7w gestation karyotype-normal pregnancy. The Negative Control used was KLH antibody. Images were taken at 400x magnification, bars represent 60\u00b5m. Figure legend: VC: villus core, CT: cytotrophoblast, ST: syncytiotrophoblast, MB: maternal blood space bathing placental villi. Image was kindly submitted by a researcher via the Antibodies Online Independent Validation Program.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CD200R2 Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7351R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-7351R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-7351R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-7351R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-7351R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Antigen: bs7518P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in Microplate Reader by 450nm\\n
Lane 1: mouse intestine lysates Lane 2: mouse brain lysates probed with Anti Ribonuclease Inhibitor Polyclonal Antibody, Unconjugated (bs-7518R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.

Catalog #1: bs-7518R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bs-7518R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bs-7518R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bs-7518R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bs-7518R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Formalin-fixed and paraffin embedded rat heart tissue labeled with Anti-PGC1 alpha Polyclonal Antibody, Unconjugated (bs-7535R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 pig muscles lysates L2 mouse kidney lysates probed with Anti PGC1 alpha Polyclonal Antibody, Unconjugated (bs-7535R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 91-110kD. Observed band size: 92kD.
Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A431 cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human HL-60 cell lysates probed with PGC1 alpha + beta Polyclonal Antibody, Unconjugated (bs-7535R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-7535R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-7535R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-7535R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-7535R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: FITC

Catalog #1: bs-7535R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-7541R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Lane 1: Mouse Heart lysates probed with Anti-EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:300 in dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Rat Cerebrum cell lysates; Lane 2: Mouse Cerebrum cell lysates; Lane 3:Rat Heart cell lysates; Lane 4: Mouse Heart cell lysates probed with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with EDG3 Polyclonal Antibody, Unconjugated (bs-7541R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7541R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-7541R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-7541R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-7541R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Paraformaldehyde-fixed (4%) Human Endothelial Cells were blocked with normal goat serum for 20mins at 37\u2103. The cells were then labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:50, overnight at 4\u00b0C. Secondary antibody incubation was performed with Goat Anti-Rabbit IgG, Rhodamine B conjugated antibody (bs-0295G-RBITC) at a 1:200 dilution for 40 minutes at 37\u00b0C. DAPI (5ug\/ml; blue) was used to stain the cell nuclei
Formalin-fixed and paraffin-embedded Human Lung Carcinoma labeled with Biglycan Polyclonal Antibody, Unconjugated (bs-7552R) at 1:200 followed by secondary antibody, DAB and counterstaining
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Biglycan Polyclonal Antibody (bs-7552R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Mouse Bone probed with Biglycan Polyclonal Antibody (bs-7552R) at 1:300, overnight at 4˚C. Followed by incubation with a conjugated secondary antibody.

Catalog #1: bs-7552R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-7552R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-7552R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-7552R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-7552R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Galactosidase alpha Polyclonal Antibody, Unconjugated (bs-7593R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: mouse liver lysates Lane 2: human colon carcinoma lysates probed with Anti Galactosidase alpha Polyclonal Antibody, Unconjugated (bs-7593R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 44kD. Observed band size: 44kD.

Catalog #1: bs-7593R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated