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Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37\u00b0C. DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining.
Lane 1: Rat Thymus lysates; Lane 2: Rat lymph node lysates probed with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4791R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Unconjugated

Catalog #1: bs-4791R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy5

Catalog #1: bs-4791R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy3

Catalog #1: bs-4791R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: Cy7

Catalog #1: bs-4791R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat, Chicken

Conjugate: FITC

Mouse spleen lysate probed with Anti-CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Human MOLT4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4815R

Applications: WB, ELISA, IHC-P, IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-4817R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

probed with Anti-CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Mouse Thymus Lane 2: Mouse embryo probed with Anti-CD4 Polyclonal Antibody (bs-4817R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Lane 1: Rat spleen cell lysates; Lane 2: Mouse spleen cell lysates; Lane 3: Rat Lymph node cell lysates; Lane 4: Mouse Lymph node cell lysates ; Lane 5: Rat Thymus cell lysates;Lane 6:Mouse Thymus cell lysates; Lane 7: Rat Small intestine cell lysates; Lane 8: Mouse Small intestine cell lysates; Lane 9: Rat Large intestine cell lysates; Lane 10: Mouse Large intestine cell lysates ; Lane 11: HL60 cell lysates;Lane 12: Jurkat cell lysates probed with CD4 Polyclonal Antibody, Unconjugated (bs-4817R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4817R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4817R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-4817R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4817R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4819R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4819R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4819R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-4819R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4819R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-4819R-PE

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE

Mouse splenocytes probed with Rabbit Anti-CD83 Polyclonal Antibody, PE Conjugated (bs-4826R) (green) at 1:20 for 40 minutes compared to control cells (blue).
A431 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD83 Antibody\uff08bs-4826R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

Catalog #1: bs-4826R

Applications: WB, ELISA, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4826R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-4826R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-4826R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-4826R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-4834R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Unconjugated

Catalog #1: bs-4834R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy5

Catalog #1: bs-4834R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy3

Catalog #1: bs-4834R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: Cy7

Catalog #1: bs-4834R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: FITC

Catalog #1: bs-4834R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep

Conjugate: PE

Catalog #1: bs-4886R

Applications: WB, ELISA, FCM, IHC-P, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4886R-FITC

Applications: WB, FCM, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4886R-Cy3

Applications: WB, FCM, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-4886R-Cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4886R-Cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

U-937 cells were stained with CD24 Polyclonal Antibody(bs-4891R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates probed with CD24 Polyclonal Antibody, Unconjugated (bs-4891R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-4891R

Applications: WB, ELISA, FCM, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4894R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4894R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4894R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-4894R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-4894R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4902R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy3

cells probed with Rabbit Anti-Calponin 2 + 3 Polyclonal Antibody (bs-4902R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 for 30 minutes (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Calponin 2 + 3 Polyclonal Antibody, Unconjugated (bs-4902R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-4902R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-4902R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bs-4902R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bs-4902R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: FITC

Catalog #1: bs-4902R-PE

Applications: FCM

Reactivity: Rat

Conjugate: PE

Catalog #1: bs-4916R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-4916R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-4916R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7