RUNX2 Recombinant Antibody

$Lane 1: U937 Cells; Lane 2: Mouse spleen; Probed with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: U937 Cells; Lane 2: Mouse spleen; Probed with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with RUNX2 (3C5) Monoclonal Antibody (bsm-52672R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 57 kDa Observed MW: 64 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: LnCAP (Negative control), 2: MDA-MB-231, 3: PC-3 Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 57 kDa Observed MW: 64 kDa

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 \u03bcg Exposure time: 30 s \/ 60 s Predicted MW: 57 kDa Observed MW: 64 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:5000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: Saos-2, 2: C2C12 Protein loading quantity: 20 \u03bcg Exposure time: 30 s \/ 60 s Predicted MW: 57 kDa Observed MW: 64 kDa

$Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human U251 cell lysates probed with RUNX2 Monoclonal Antibody, Unconjugated (bsm-52672R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human U251 cell lysates probed with RUNX2 Monoclonal Antibody, Unconjugated (bsm-52672R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52672r-100ul
Product Name	RUNX2 Recombinant Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human RUNX2 between 300 to 450 amino acids
Immunogen Range	300-450/521
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	860
Swiss Prot	Q13950
Synonyms	Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, RUNX2
Background	Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC