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Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:300 followed by conjugation to the secondary PE antibody and DAB staining
Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pro-Collagen 1 Polyclonal Antibody, Unconjugated (bs-0578R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-0578R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Fish

Conjugate: Unconjugated

Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-DKK1 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Image kindly submitted by Dr. Magdalena Krol. Formalin-fixed and paraffin embedded canine mammary tumor labeled with Rabbit Anti-DKK1 Polyclonal Antibody, Unconjugated (bs-2162R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-2162R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Unconjugated

Catalog #1: bs-4729R

Applications: WB, ELISA

Reactivity: Human, Mouse, Dog

Conjugate: Unconjugated

Catalog #1: bs-0014R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0014R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-0014R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-2162R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: Biotin

Catalog #1: bs-0014R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0014R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0014R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-2162R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy3

Catalog #1: bs-2162R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5

Catalog #1: bs-2162R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy7

Catalog #1: bs-2162R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: FITC

Catalog #1: bs-2162R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Dog

Conjugate: HRP

Catalog #1: bs-4729R-Biotin

Applications: WB, ELISA

Reactivity: Human, Mouse, Dog

Conjugate: Biotin

Catalog #1: bs-4729R-HRP

Applications: WB, ELISA

Reactivity: Human, Mouse, Dog

Conjugate: HRP

Catalog #1: bs-0014R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5.5

Catalog #1: bs-2162R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Dog

Conjugate: Cy5.5

Catalog #1: bs-0014R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0014R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0014R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0014R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0014R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0014R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0014R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0014R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0014R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC

Catalog #1: bs-0014R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy5.5

Catalog #1: bs-0014R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: APC-Cy7

Catalog #1: bs-0014R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP

Catalog #1: bs-0014R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0014R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: RBITC

Catalog #1: bs-0014R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0014R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5

Catalog #1: bs-0014R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy5.5

Catalog #1: bs-0014R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE-Cy7

Catalog #1: bs-4729R-Cy3

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: Cy3

Catalog #1: bs-4729R-Cy5

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: Cy5

Catalog #1: bs-4729R-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: Cy5.5

Catalog #1: bs-4729R-Cy7

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: Cy7

Catalog #1: bs-4729R-FITC

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: FITC

Catalog #1: bs-4729R-PE

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: PE

Catalog #1: bs-4729R-BF350

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor® 350

Catalog #1: bs-4729R-BF405

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor® 405

Catalog #1: bs-4729R-BF488

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor® 488

Catalog #1: bs-4729R-BF555

Applications: WB

Reactivity: Human, Mouse, Dog

Conjugate: AbBy Fluor® 555