Cathepsin D/CTSD Polyclonal Antibody

Formalin-fixed and paraffin embedded human skin of vulva tissue labeled with Anti-Cathepsin D Polyclonal Antibody, Unconjugated (bs-1615R) at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining

$Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Cathepsin D\/CTSD Polyclonal Antibody (bs-1615R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.$

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Cathepsin D\/CTSD Polyclonal Antibody (bs-1615R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

$Lane 1: Rat Liver tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with Cathepsin D Polyclonal Antibody, Unconjugated (bs-1615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Liver tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with Cathepsin D Polyclonal Antibody, Unconjugated (bs-1615R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-1615R
Product Name	Cathepsin D/CTSD Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Capsin D
Immunogen Range	101-200/412
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1509
Swiss Prot	P07339
Subcellular location	Cytoplasm, Secreted
Synonyms	CPSD; CLN10; HEL-S-130P; Cathepsin D; CTSD
Background	Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200