Granulin Polyclonal Antibody

$Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti PGRN\/Granulin Polyclonal Antibody,Unconjugated (bs-0823R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Anti PGRN\/Granulin Polyclonal Antibody,Unconjugated (bs-0823R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti alpha Actinin PGRN\/Granulin Polyclonal Antibody, Unconjugated (bs-0823R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 63kD. Observed band size: 63kD.$

Lane 1: mouse brain lysates Lane 2: human colon carcinoma lysates probed with Anti alpha Actinin PGRN\/Granulin Polyclonal Antibody, Unconjugated (bs-0823R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 63kD. Observed band size: 63kD.

$Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Granulin) Polyclonal Antibody, Unconjugated (bs-0823R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Granulin) Polyclonal Antibody, Unconjugated (bs-0823R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-0823R
Product Name	Granulin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This antibody is specific for Granulin-6
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Granulin
Immunogen Range	451-550/593
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2896
Swiss Prot	P28799
Subcellular location	Secreted
Synonyms	GEP; GP88; PEPI; PGRN; CLN11; PCDGF; Granulins; Proepithelin; GRN
Background	Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200