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Neurodegenerative Disease

Neurodegenerative Disease


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Formalin-fixed and paraffin embedded rat brain labeled with Anti-UCHL1\/PGP9.5 Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (UCHL1\/PGP9.5) Polyclonal Antibody, Unconjugated (bs-3806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-3806R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates, L2 rat brain lysates probed : Anti-GDF9 (bs-2951R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 48kDa and 62kD
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-2951R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Hsc70 Polyclonal Antibody (bs-5117R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Hsc70 Polyclonal Antibody(bs-5117R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
NIH\/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Hsc70) polyclonal Antibody, Unconjugated (bs-5117R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-5117R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Cow

Conjugate: Unconjugated

Catalog #1: bs-2951R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #1: bs-3806R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Cy3

Catalog #1: bs-3806R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: FITC

Catalog #1: bs-2951R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #1: bs-2951R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #1: bs-2951R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #1: bs-2951R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #1: bs-2951R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #1: bs-3806R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Cy5

Catalog #1: bs-3806R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Cy7

Catalog #1: bs-3806R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Biotin

Catalog #1: bs-3806R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: HRP

Catalog #1: bs-3806R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: Cy5.5

Catalog #1: bs-2951R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5.5

Catalog #1: bs-3806R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PE

Catalog #1: bs-3806R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 350

Catalog #1: bs-3806R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 405

Catalog #1: bs-3806R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 488

Catalog #1: bs-3806R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 555

Catalog #1: bs-3806R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 594

Catalog #1: bs-3806R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 647

Catalog #1: bs-3806R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 680

Catalog #1: bs-3806R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: AbBy Fluor® 750

Catalog #1: bs-3806R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: APC

Catalog #1: bs-3806R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: APC-Cy5.5

Catalog #1: bs-3806R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: APC-Cy7

Catalog #1: bs-3806R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PE-Cy5

Catalog #1: bs-3806R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PE-Cy5.5

Catalog #1: bs-3806R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PE-Cy7

Catalog #1: bs-3806R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PerCP

Catalog #1: bs-3806R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3806R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Goat, Cow

Conjugate: RBITC

Catalog #1: bs-2951R-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #1: bs-2951R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 350

Catalog #1: bs-2951R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 405

Catalog #1: bs-2951R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 488

Catalog #1: bs-2951R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 555

Catalog #1: bs-2951R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 594

Catalog #1: bs-2951R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 647

Catalog #1: bs-2951R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 680

Catalog #1: bs-2951R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: AbBy Fluor® 750

Catalog #1: bs-2951R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC

Catalog #1: bs-2951R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy5.5

Catalog #1: bs-2951R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: APC-Cy7

Catalog #1: bs-2951R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE-Cy5