CD3G Recombinant Antibody

$Lane 1: Mouse Thymus; Probed with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Thymus; Probed with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of Jurkat cells with CD3G (2B11) Monoclonal Antibody (bsm-54300R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates probed with CD3 gamma Monoclonal Antibody, Unconjugated (bsm-54300R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates probed with CD3 gamma Monoclonal Antibody, Unconjugated (bsm-54300R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-54300r-100ul
Product Name	CD3G Recombinant Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within C-terminal human CD3G
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	917
Swiss Prot	P09693
Subcellular location	Cell membrane
Synonyms	T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; CD3g; CD3G; T3G
Background	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface (PubMed:8187769). Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400