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Musculoskeletal Biology

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Catalog #1: bsm-51559M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Lane 1: Raji cell lysates probed with HLA-F Monoclonal Antibody, Unconjugated (bsm-51712M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates; Lane 2: HL-60 cell lysates probed with HLA-F Monoclonal Antibody, Unconjugated (bsm-51712M) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51712M

Applications: WB

Reactivity:

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 3 s\r\nPredicted MW: 23 kDa\r\nObserved MW: 23 kDa
Tissue:rat spleen\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60002R
Tissue:mouse spleen\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60002R
Tissue:Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60002R

Catalog #1: bsm-60002R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Tissue: Human spleen Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60605R
Tissue: Human tonsil Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60605R
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: Ramos, 2: Daudi, 3: Raji\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 61 kDa\r\nObserved MW: 95 kDa\r\n
Cell line: Raji\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60605R

Catalog #1: bsm-60605R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Mouse spleen\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 33 kDa
Tissue:Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60290R
Tissue:Human lymphoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60290R

Catalog #1: bsm-60290R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nLysate: Mouse spleen, Raji\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 29 kDa\r\nObserved MW: 29 kDa
Tissue:Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60291R

Catalog #1: bsm-60291R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP)\r\nLysate: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 20 kDa\r\nObserved MW: 18-28 kDa
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60390R
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60390R
Lane 1: Human Jurkat cell Lysates; Lane 2: Human MOLT4 cell Lysates. Probed with CD3G monoclonal Antibody, unconjugated (bsm-60390R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60390R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30092M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30092M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30092M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30092M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30092M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30092M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30092M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30094M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30094M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30094M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30094M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30094M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30094M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30094M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30096M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30096M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30096M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30096M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30096M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30096M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30096M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30098M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30098M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30098M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30098M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30098M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30098M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30098M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30396M

Applications: FCM

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60508M

Applications: WB, IHC-P, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60283M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2459R-Biotin

Applications: WB, ELISA

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-60628M

Applications: FCM, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-2459R-HRP

Applications: WB, ELISA

Reactivity:

Conjugate: HRP

Catalog #1: bs-0480R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0480R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0480R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0480R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0480R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0480R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0480R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor® 680