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Musculoskeletal Biology

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THP-1 cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Raw264.7 cells were treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation).The cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at at room temperature and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TNF alpha Polyclonal Antibody(bs-41192R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-41192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-41192R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-41192R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HLA F Monoclonal Antibody, Unconjugated (bsm-54713R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HLA F Monoclonal Antibody, Unconjugated (bsm-54713R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54713R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0080R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0765R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-0753r-cy3-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0753r-cy5-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0753r-cy55-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-0753r-cy7-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0753r-fitc-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1279R-PE

Applications: WB, FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bs-1459R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-10802R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10802R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-10802R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-10802R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-20639R-Cy3

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-20639R-Cy7

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-20639R-Cy5.5

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-20639R-FITC

Applications: WB, FCM

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-20639R-PE

Applications: WB, FCM

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-20639R-Cy5

Applications: WB, FCM

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-41192R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-41192R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-41192R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bs-41192R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-41192R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-20782R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Sheep

Conjugate: Cy3

Catalog #1: bs-20782R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Sheep

Conjugate: Cy5.5

Catalog #1: bs-20782R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Sheep

Conjugate: Cy7

Catalog #1: bs-0480R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-10498R-PE

Applications: FCM

Reactivity: Human, Mouse, Pig

Conjugate: PE

Catalog #1: bs-1805R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bs-4791R-PE

Applications: WB, FCM

Reactivity: Human, Rat, Chicken

Conjugate: PE

Catalog #1: bs-4815R-Cy3

Applications: WB

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-4815R-PE

Applications: WB

Reactivity: Mouse

Conjugate: PE

Catalog #1: bs-4815R-Cy5.5

Applications: WB

Reactivity: Mouse

Conjugate: Cy5.5

Catalog #1: bs-4815R-Cy7

Applications: WB

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-4815R-FITC

Applications: WB

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-4815R-Cy5

Applications: WB

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-20373R-Cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-20373R-FITC

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-20373R-Cy5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-20373R-Cy5.5

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bs-20373R-Cy7

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-20373R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-51506M

Applications: WB

Reactivity:

Conjugate: Unconjugated