Tropomyosin 1 (2G2) Monoclonal Antibody

$Lane 1: Rat Heart; Probed with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Heart; Probed with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Rat Smooth Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Smooth Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Flow cytometric analysis of MCF-7 cells with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at a 1:100 dilution (purple) compared with an unlabeled control (cells without incubation with primary antibody; yellow).

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54406R
Product Name	Tropomyosin 1 (2G2) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Tropomyosin 1 aa 100-300.
Clonality	Monoclonal
Clone #	2G2
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7168
Swiss Prot	P09493
Subcellular location	Cytoskeleton
Synonyms	Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1; TPM1; C15orf13; TMSA
Background	Tropomyosins are a group of structural proteins. Tropomyosins are present in virtually all eukaryotic cells, both muscle and non-muscle, where they bind Actin filaments and function to modulate Actin-Myosin interaction and stabilize Actin filament structure. Tropomyosin _ is encoded by the TPM1 gene, which maps to human chromosome 15q22.1 and undergoes alternative splicing to generate at least four isoforms, including skeletal muscle (isoform 1), smooth muscle (isoform 2), fibroblast/TM3 (isoform 3) and isoform 4. Troponin I binds Tropomyosin at a specific region and the association of Tropomyosin-Troponin with Actin filaments may increase the rigidity of Actin filaments. Tropomyosin also interacts with Caldesmon to regulate smooth muscle contraction.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400