GLTSCR2/PICT1 Polyclonal Antibody

$Mouse pancreas lysates probed with GLTSCR2\/PICT1 Polyclonal Antibody, Unconjugated (bs-13382R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Mouse pancreas lysates probed with GLTSCR2\/PICT1 Polyclonal Antibody, Unconjugated (bs-13382R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with GLTSCR2 Polyclonal Antibody, Unconjugated (bs-13382R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human MCF-7 cell lysates; Lane 2: Human HeLa cell lysates probed with GLTSCR2 Polyclonal Antibody, Unconjugated (bs-13382R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-13382R
Product Name	GLTSCR2/PICT1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GLTSCR2/PICT1
Immunogen Range	401-478/478
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	29997
Subcellular location	Nucleus
Synonyms	Glioma tumor suppressor candidate region gene 2 protein; GLTSCR2; GSCR2_HUMAN; p60; PICT1; protein interacting with carboxyl terminus 1.
Background	The tumor suppressor PTEN plays an essential role in regulating signaling pathways involved in cell growth and apoptosis and is inactivated in a wide variety of tumors. Protein interacting with PTEN carboxyl terminus 1 (PICT-1), also designated p60 or Glioma tumor suppressor candidate region gene 2 protein, binds to the C-terminus of PTEN and regulates its turnover. Five Ser/Thr residues within the C-terminal segment of PTEN, including Ser-380, are phosphorylated upon binding of PTEN to PICT-1 and may contribute to the stabilization of PTEN. PICT-1 is localized to the nucleus and/or nucleolus and is highly expressed in pancreas and heart, but can also be detected in liver, skeletal muscle, placenta and kidney. PICT-1 also interacts with herpes simplex virus 1 regulatory proteins ICP22 and ICP0. The tumor suppressor GLTSCR2 gene, which encodes PICT-1, is located in a 150-kb minimal common deletion region for human gliomas, especially oligodendrogliomas, and maps to human chromosome 19q13.3.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500