Girdin (Tyr1765) Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Girdin (Tyr1765) Polyclonal Antibody, Unconjugated (bs-13358R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Girdin (Tyr1765) Polyclonal Antibody, Unconjugated (bs-13358R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Overview
Catalog #	bs-13358R
Product Name	Girdin (Tyr1765) Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human APE around the phosphorylation site of Tyr1765
Modification Site	Tyr1765
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55704
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Girdin phospho Y1765; p-Girdin phospho Y1765; Akt phosphorylation enhancer; APE; Coiled coil domain containing protein 88A; G alpha interacting vesicle associated protein; Girders of actin filament; GIV; HkRP1; Hook related protein 1; AKT iphosphorylation enhancer; Akt phosphorylation enhancer; Ccdc88a; GIV; GRDN; GRDN_HUMAN; HkRP1; Hook related protein 1; Hook-related protein 1; KIAA1212.
Background	Girdin is a 1,871 amino acid protein that, via interactions with phosphoinositides, localizes to the cell membrane and to the cytosol. Expressed ubiquitously, girdin functions to enhance the activity of PI 3-kinase-dependent phosphorylation of proteins, such as Akt1, thereby inducing the phosphorylation of downstream protein targets and, ultimately, regulating DNA replication and cellular proliferation. Additionally, girdin is crucial for cell migration and is responsible for both maintaining the structural integrity of the Actin cytoskeleton and for regulating the formation of Actin stress fibers. Girdin exists as a homodimer that can itself be phosphorylatedan event that delocalizes girdin from the cell membrane, thus allowing it to participate in cell migration events. Two isoforms of girdin exist due to alternative splicing events.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500