SDHB Recombinant Antibody

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Flow cytometric analysis of Jurkat cells with SDHB (4C7) Monoclonal Antibody (bsm-54180R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver Cancer tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat Esophagus tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat Esophagus tissue incubated with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Lane 1: Human Liver; Lane 2: Rat Spleen; Lane 3: Rat Liver; Lane 4: Mouse Spleen; lane 5: HepG2 Cells; Probed with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Liver; Lane 2: Rat Spleen; Lane 3: Rat Liver; Lane 4: Mouse Spleen; lane 5: HepG2 Cells; Probed with SDHB (4C7) Monoclonal Antibody (bsm-54180R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54180r-100ul
Product Name	SDHB Recombinant Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human SDHB aa 1-150
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	6390
Swiss Prot	P21912
Subcellular location	Mitochondrion
Synonyms	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Iron-sulfur subunit of complex II, Ip, SDHB, SDH, SDH1
Background	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400