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Loading Controls

Loading Controls

 

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples.

Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.



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Catalog #1: bsm-0945M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0945M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-33128M-HRP

Applications: WB

Reactivity: Plant

Conjugate: HRP

Catalog #1: bsm-0945M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33128M-Biotin

Applications: WB

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bsm-8770M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-8772M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-33128M

Applications: WB

Reactivity: Plant

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated