phospho-AKT (Thr308) Recombinant Antibody

$Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: NIH\/3T3, 2: NIH\/3T3 + PDGF (100\r\nng\/mL, 15 min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 56 kDa Observed MW: 60 kDa$

Blocking buffer: 5% NFDM\/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L\r\n(HRP) Lysate: 1: NIH\/3T3, 2: NIH\/3T3 + PDGF (100\r\nng\/mL, 15 min) Protein loading quantity: 20 \u03bcg Exposure time: 60 s Predicted MW: 56 kDa Observed MW: 60 kDa

Applications

Predicted Reactivity

Mouse

Overview
Catalog #	bsm-60645r-100ul
Product Name	phospho-AKT (Thr308) Recombinant Antibody
Applications	WB
Predicted Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-AKT (Thr308)
Modification Site	Thr308
Clonality	Recombinant
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	208
Swiss Prot	P31751
Synonyms	AKT1 (phospho T308); AKT2 (phospho T309); AKT3 (phospho T305); p-AKT1 (phospho T308); p-AKT2 (phospho T309); p-AKT3 (phospho T305); AKT 1; AKT; AKT1; AKT-1; AKT1_HUMAN; AKT 2; AKT2; AKT-2; AKT2_HUMAN; AKT 3; AKT3; AKT-3; AKT3_HUMAN; C AKT; cAKT; MGC9965; MGC99656; Oncogene AKT1; PKB; PKB alpha; PKB-ALPHA; PKB beta; PKB gamma; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC alpha serine/threonine protein kinase; RAC; RAC PK Alpha; Rac protein kinase alpha; RAC Serine/Threonine Protein Kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; v akt murine thymoma viral oncogene homolog 1; vAKT Murine Thymoma Viral Oncogene Homolog 1.
Application Dilution
WB	1:300-5000