NFKB1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB1 Polyclonal Antibody, Unconjugated (bs-55152R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB1 Polyclonal Antibody, Unconjugated (bs-55152R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Hela cell lysates; Lane 2: NFKB1 knockout (KO) HeLa cell lysates probed with NFKB1 Polyclonal Antibody, Unconjugated (bs-55152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Raji cell lysates; Lane 2: Mouse Thymus lysates probed with NFKB1 Polyclonal Antibody, Unconjugated (bs-55152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n$

Lane 1: Raji cell lysates; Lane 2: Mouse Thymus lysates probed with NFKB1 Polyclonal Antibody, Unconjugated (bs-55152R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n\r\n

$A547 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NFKB1) polyclonal Antibody, Unconjugated (bs-55152R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

A547 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NFKB1) polyclonal Antibody, Unconjugated (bs-55152R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(ICC)
IHC
IF

Reactivity

Human
Mouse

Overview
Catalog #	bs-55152R
Product Name	NFKB1 Polyclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC, IF
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4790
Swiss Prot	P19838
Subcellular location	Cytoplasm, Nucleus
Synonyms	NFKB1;CVID12;EBP-1;KBF1;NF-kB1;NF-kappa-B;NF-kappaB;NFKB-p105;NFKB-p50;NFkappaB;p105;p50
Background	This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC
IF