TLR2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).

$Mouse spleen cells probed with \\tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

Mouse spleen cells probed with \\tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Lane 1: Mouse Spleen; Lane 2: Mouse Thymus; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse Spleen; Lane 2: Mouse Thymus; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Raw264.7 Cells; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Raw264.7 Cells; Probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:300 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Lane 1: Cerebrum lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Rat Spleen lysates; Lane 2: Rat Bronchus lysates; Lane 3: Rat Lung lysates probed with TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IHC-F
IF(ICC)

Reactivity

Human
Mouse
Rat
Bovine
Pig
Sheep

Predicted Reactivity

Dog
Cow
Chicken

Overview
Catalog #	bs-1019R
Product Name	TLR2 Polyclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(ICC)
Reactivity	Human, Mouse, Rat, Bovine, Pig, Sheep
Predicted Reactivity	Dog, Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TLR2 (cytoplasmic domain)
Immunogen Range	701-784/784
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7097
Swiss Prot	O60603
Subcellular location	Cell membrane
Synonyms	TIL4; CD282; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; TLR2
Background	Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200