GM-CSF Polyclonal Antibody

Formalin-fixed and paraffin embedded rat aortic cavity labeled with Rabbit Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat intestine tissue labeled with Anti-GM-CSF Polyclonal Antibody (bs-0999R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Lane 1: GM-CSF+Trx\/His-tag fusion protein\uff1bLane 2: GM-CSF fusion protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-0999R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: GM-CSF+Trx\/His-tag fusion protein\uff1bLane 2: GM-CSF fusion protein probed with GM-CSF Polyclonal Antibody, Unconjugated (bs-0999R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bs-0999R
Product Name	GM-CSF Polyclonal Antibody
Applications	WB
Reactivity	Pig
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse GM-CSF
Immunogen Range	71-141/141
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12981
Swiss Prot	P01587
Subcellular location	Secreted
Synonyms	Granulocyte-Macrophage Colony Stimulating Factor; Burst Promoting Activity; CMCSF; Colony Stimulating Factor 2; Colony stimulating factor; CSF 2; CSF Alpha; CSF; CSF2; Eosinophil Colony Stimulating Factor; GMCSF; Granulocyte Macrophage Colony Stimulating Factor; MGC131935; MGC138897; Molgramostin; Pluripoietin Alpha; Sargramostim; granulocyte-macrophage colony-stimulating factor precursor; CSF2_HUMAN; CSF2_MOUSE.
Background	Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Application Dilution
WB	1:300-5000