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Human CRIM1 Ready-To-Use IHC Kit

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human colon cancer tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human kidney tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human placenta tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human pancreas tissue slide using IHC0202H (Human CRIM1 IHC Kit).
Immunohistochemical analysis of paraffin embedded human pancreatic cancer tissue slide using IHC0202H (Human CRIM1 IHC Kit).

Human CRIM1 Ready-To-Use IHC Kit

Cat. No.: IHC0202H
Sample Type: FFPE tissue
Size: 50T (including a control slide)
Storage and Stability: Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Background

CRIM1 (cysteine-rich motor neuron 1), a glycosylated type I transmembrane protein, plays a role in tissue development i.e. capillary formation and maintenance during angiogenesis. It contains an N-terminal IGF-binding protein-like motif and six von Willebrand-like cysteine-rich repeats (CRRs) in its extracellular domain. CRIM1 interacts with BMP4 and BMP7 via the CRRs and functions as an antagonist. CRIM1 is developmentally expressed in a number of tissues including the pancreas, kidney, placenta, brain and blood vessels. CRIM1 may participate in CNS and placental development by interacting with growth factors involved in motor neuron differentiation and survival.

Synonyms

Cysteine-rich motor neuron 1 protein, CRIM-1, Cysteine-rich repeat-containing protein S52, Processed cysteine-rich motor neuron 1protein, CRIM 1, CRIM1, Cysteine rich motor neuron 1 protein, Cysteine rich repeat containing protein S52, Cysteine rich transmembrane BMP regulator 1 (chordin like), Cysteine rich transmembrane BMP regulator 1, MGC138194, Processed cysteine rich motor neuron 1 protein, S52.

Materials Supplied

Number Component 50T Concentration Storage
1 PBS Buffer (powder) 2L x 2 20x RT
2 Antigen Retrieval Buffer 20ml 100x 2-8°C
3 Endogenous Peroxidase Blocking Buffer 3ml RTU 2-8°C
4 Blocking Buffer 3ml RTU 2-8°C
5 Primary Antibody (Human CRIM1 Rabbit pAb) 6ml RTU 2-8°C
6 Secondary Antibody (Goat Anti-Rabbit IgG H&L / HRP) 6ml RTU 2-8°C
7 Chromogen Component A 0.3ml RTU -20°C
8 Chromogen Component B 0.3ml RTU -20°C
9 Counter Staining Reagent 5ml RTU RT
10 Differentiation Reagent 6ml RTU RT
11 Mounting Media 5ml RTU RT
12 Control slide (Human placenta) 1 slide RTU RT
13 Datasheet 1 copy

Notes

  1. The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
  2. Do not allow slides to dry at any time during this procedure.
  3. Please don't replace the matching reagents in this product with other manufacturers' products.
  4. As DAB is a carcinogen, please take necessary precautions.
  5. PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.


As DAB is a carcinogen, please take necessary precautions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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