LDHD Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human colon colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LDHD Monoclonal Antibody, Unconjugated (bsm-54704R) at 1:200 for 30 minutes at room temperature, DAB staining.

$HT-29 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:100, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

HT-29 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:100, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:50, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:50, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

$Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:50, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.$

Siha cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (LDHD) Monoclonal Antibody, Unconjugated (bsm-54704R) 1:50, 90 minutes at 37\u00b0C;followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54704R
Product Name	LDHD Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic Peptide within N-terminal human LDHD.
Clonality	Monoclonal
Clone #	1H51
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	197257
Swiss Prot	Q86WU2
Subcellular location	Mitochondrion
Synonyms	D lactate dehydrogenase antibody, DLD antibody, Lactate dehydrogenase D antibody, Ldhd antibody, LDHD_HUMAN antibody, MGC57726 antibody, mitochondrial antibody, Probable D lactate dehydrogenase mitochondrial antibody, Probable D-lactate dehydrogenase antibody
Background	LDHD belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. D-lactate and D-glycerate dehydrogenase activities have been found in a similar protein in yeast. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200