Arginase-1 (2C12) Monoclonal Antibody

$Lane 1: Human Liver; Probed with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Human Liver; Probed with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Arginase-1 (2C12) Monoclonal Antibody (bsm-54331R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54331R
Product Name	Arginase-1 (2C12) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human Liver Arginase aa 100-300
Clonality	Monoclonal
Clone #	2C12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	383
Swiss Prot	P05089
Subcellular location	Cytoplasm
Synonyms	Arginase-1; Liver Arginase; Type I arginase; ARG1
Background	Arginase I which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea . The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster. Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location. Specifically, arginase II localizes to the mitochondria. Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver. The human arginase II gene, which maps to chromosome 14q24.1-q24.3, encodes a 354 amino acid protein. In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400