AFP Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HepG2 cell lysate probed with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysate probed with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human Fetal Liver tissue lysates probed with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Fetal Liver tissue lysates probed with AFP Monoclonal Antibody, Unconjugated (bsm-51467M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P

Predicted Reactivity

Human

Overview
Catalog #	bsm-51467M
Product Name	AFP Monoclonal Antibody
Applications	WB, IHC-P
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human AFP
Immunogen Range	281-609/609
Clonality	Monoclonal
Clone #	A01H1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	174
Swiss Prot	P02771
Subcellular location	Secreted
Synonyms	Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
Application Dilution
WB	=1:500ÔºåIHC-P=1:25ÔºåIHC-F=1:25Ôºå
IHC-P	1:200-400