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Thrombin heavy chain Polyclonal Antibody, HRP Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Fish
Overview
Catalog # bs-1914R-HRP
Product Name Thrombin heavy chain Polyclonal Antibody, HRP Conjugated
Applications ELISA, IHC-P, IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Chicken, Rabbit, Fish
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Thrombin heavy chain
Immunogen Range 551-622/622
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2147
Subcellular location Secreted, Extracellular matrix
Synonyms coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II thrombin; Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin; Thrombin heavy chain.
Background Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500