Plasminogen Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plasminogen Polyclonal Antibody, Unconjugated (bs-1828R) at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Plasminogen Polyclonal Antibody, Unconjugated (bs-1828R) at 1:400 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

Applications

WB
ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig

Overview
Catalog #	bs-1828R
Product Name	Plasminogen Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLG
Immunogen Range	251-350/810
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5340
Swiss Prot	P00747
Subcellular location	Secreted, Cell membrane, Extracellular matrix
Synonyms	Plasminogen; Angiostatin; DKFZp779M0222; Microplasmin; Plasmin; Plasmin heavy chain A; PLG; PLMN_HUMAN.
Background	Plasminogen is a plasma protein synthesised mostly in the liver. Plasma concentration is usually in the range of 70 to 200mg/l. Plasminogen is activated by tissue plasminogen activator, urokinase and streptokinase, to form plasmin. Activation results from the cleavage and release of the preactivation peptide. Angiostatin, an internal fragment of plasminogen, is a potent inhibitor of angiogenesis, which selectively inhibits endothelial cell proliferation. Angiostatin potently inhibits tumor growth and can maintain metastatic and primary tumors in a dormant state. It is defined by a balance of proliferation and apoptosis of the tumor cells and is composed of plasminogen first four cringle structures. This molecule is generated by proteolytic cleavage of plasminogen.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400